dc.contributor.author | Islam, Rakibul | |
dc.contributor.author | Eidet, Jon Roger | |
dc.contributor.author | Badian, Reza Ario | |
dc.contributor.author | Lippestad, Marit | |
dc.contributor.author | Messelt, Edvard Berger | |
dc.contributor.author | Griffith, May | |
dc.contributor.author | Dartt, Darlene Ann | |
dc.contributor.author | Utheim, Tor Paaske | |
dc.date.accessioned | 2018-05-04T11:53:10Z | |
dc.date.available | 2018-05-04T11:53:10Z | |
dc.date.created | 2017-08-31T10:20:41Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Scientific Reports. 2017, 7:674 1-12. | nb_NO |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/11250/2497201 | |
dc.description.abstract | Transplantation of cultured oral mucosal epithelial cells (OMECs) is a promising treatment strategy for limbal stem cell deficiency. In order to improve the culture method, we investigated the effects of four culture media and tissue harvesting sites on explant attachment, growth, and phenotype of OMECs cultured from Sprague-Dawley rats. Neither choice of media or harvesting site impacted the ability of the explants to attach to the culture well. Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM) and Roswell Park Memorial Institute 1640 medium (RPMI) supported the largest cellular outgrowth. Fold outgrowth was superior from LL explants compared to explants from the buccal mucosa (BM), HP, and transition zone of the lower lip (TZ) after six-day culture. Putative stem cell markers were detected in cultures grown in DMEM and RPMI. In DMEM, cells from TZ showed higher colony-forming efficiency than LL, BM, and HP. In contrast to RPMI, DMEM both expressed the putative stem cell marker Bmi-1 and yielded cell colonies. Our data suggest that OMECs from LL and TZ cultured in DMEM give rise to undifferentiated cells with high growth capacity, and hence are the most promising for treatment of limbal stem cell deficiency. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.relation.uri | https://www.nature.com/articles/s41598-017-00417-z | |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.subject | Oral mucosal epithelial cells | nb_NO |
dc.subject | OMECs | nb_NO |
dc.subject | epithelium | nb_NO |
dc.subject | limbal tissue | nb_NO |
dc.subject | stem cells | nb_NO |
dc.subject | transplantation | nb_NO |
dc.subject | donors | nb_NO |
dc.subject | limbus | nb_NO |
dc.subject | tissue harvesting | nb_NO |
dc.subject | limbal stem cell deficiency | nb_NO |
dc.subject | treatment | nb_NO |
dc.title | Tissue harvesting site and culture medium affect attachment, growth, and phenotype of ex vivo expanded oral mucosal epithelial cells | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.rights.holder | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | nb_NO |
dc.source.pagenumber | 1-12 | nb_NO |
dc.source.volume | 7:674 | nb_NO |
dc.source.journal | Scientific Reports | nb_NO |
dc.identifier.doi | 10.1038/s41598-017-00417-z | |
dc.identifier.cristin | 1490127 | |
cristin.unitcode | 1991,2,0,0 | |
cristin.unitname | Div Elverum-Hamar | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |