dc.contributor.author | Opstad, Trine Baur | |
dc.contributor.author | Alexander, Jan | |
dc.contributor.author | Aaseth, Jan | |
dc.contributor.author | Larsson, Anders | |
dc.contributor.author | Seljeflot, Ingebjørg | |
dc.contributor.author | Alehagen, Urban | |
dc.date.accessioned | 2023-05-05T12:01:31Z | |
dc.date.available | 2023-05-05T12:01:31Z | |
dc.date.created | 2023-04-19T18:40:29Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Antioxidants. 2023, 12 (3), 1-13. | en_US |
dc.identifier.issn | 2076-3921 | |
dc.identifier.uri | https://hdl.handle.net/11250/3066466 | |
dc.description.abstract | Background: Selenium and coenzyme Q10 (SeQ10) possess antioxidant and anti-inflammatory properties, potentially mediated via Sirtuin1 (SIRT1). We aimed to investigate the influence of a SeQ10 intervention on SIRT1 concentration, with potential interactions with microRNAs. Methods: In this sub-study of a prospective double-blind placebo-controlled clinical trial, healthy subjects (mean age 76 years) were randomized to receive an active treatment (n = 165, combined 200 µg/day of Se and 200 mg/day of Q10) or a placebo (n = 161). SIRT1 concentration and microRNAs were measured with ELISA and PCR, respectively. Results: After four years, SIRT1 concentration was increased in the active treatment group, with mean (SD) ng/mL of 469 (436) vs. 252 (162), p < 0.001, and decreased in the placebo group, 190 (186) vs. 269 (172), p = 0.002, and the differences between the groups were significant (p = 0.006, adjusted). Those who suffered CV death during a 10-year follow-up (n = 25 and n = 52 in the active treatment and placebo groups, respectively) had significantly lower baseline SIRT1 concentrations compared to the survivors (p < 0.001). MiR-130a-3p was significantly downregulated during the intervention and correlated inversely with SIRT1 at baseline (r = -0.466, p = 0.007). Conclusion: The increased SIRT1 concentration after the SeQ10 intervention associated with reduced CV mortality, partly mediated via miR-1303a-3p, suggests that SIRT1 is an additional mediator of the intervention, preventing vascular ageing. | en_US |
dc.description.sponsorship | This research was funded by the Stein Erik Hagen Foundation for Clinical Heart Research, Oslo, Norway. Part of the analysis cost was funded by grants from Pharma Nord Aps, Vejle, Denmark, and the County Council of Östergötland, Linköping University, Sweden. The selenium and coenzyme Q10 tablets were provided by Pharma Nord Aps, Vejle, Denmark. | en_US |
dc.language.iso | eng | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.subject | cardiovascular mortality; | en_US |
dc.subject | coenzyme Q10; | en_US |
dc.subject | intervention; | en_US |
dc.subject | selenium; | en_US |
dc.subject | sirtuin1; | en_US |
dc.title | Increased SIRT1 Concentration Following Four Years of Selenium and Q<inf>10</inf> Intervention Associated with Reduced Cardiovascular Mortality at 10-Year Follow-Up—Sub-Study of a Previous Prospective Double-Blind Placebo-Controlled Randomized Clinical Trial | en_US |
dc.title.alternative | Increased SIRT1 Concentration Following Four Years of Selenium and Q<inf>10</inf> Intervention Associated with Reduced Cardiovascular Mortality at 10-Year Follow-Up—Sub-Study of a Previous Prospective Double-Blind Placebo-Controlled Randomized Clinical Trial | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | en_US |
dc.source.pagenumber | 1-13 | en_US |
dc.source.volume | 12 | en_US |
dc.source.journal | Antioxidants | en_US |
dc.source.issue | 3 | en_US |
dc.identifier.doi | 10.3390/antiox12030759 | |
dc.identifier.cristin | 2141943 | |
dc.source.articlenumber | 759 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |